Job Description

The Huang Lab at UCSF ( https://huanglabucsf.com/ ) investigates the genetics and biology of leukemia through the use of relevant mouse and cell line models. Specific goals include: (1) to determine the biochemical mechanisms governing growth, survival and differentiation of leukemia cells; (2) to identify genes and biochemical pathways that are regulated differentially in leukemia versus normal cells; (3) to measure the growth of distinct populations of normal and leukemia bone marrow cells in vivo and in vitro; (4) to harvest murine bone marrow and embryonic fetal livers for transplantation into irradiated recipients; (5) to transplant genetically modified hematopoietic cells into recipient mice; (6) to perform genetic analysis on mouse tissues; and, (7) to administer chemotherapeutic agents, radiation, and new experimental treatments to mice. This SRA 1 position is for an individual who will work on leukemia research in mouse models and cell lines. SRA duties will include: cell/tissue culture, biochemical assays (western blotting, immunoprecipitation, qPCR), DNA and RNA extraction and manipulation, PCR and agarose gel electrophoresis, generation of recombinant retroviruses/lentiviruses and transduction of hematopoietic cells, flow cytometry, maintenance of accurate and complete records, organization and maintenance of mouse colonies (including computerized record-keeping, breeding, monitoring and genotyping), and experimental procedures using laboratory mice (blood sampling, intravenous/intraperitoneal injection, dissection, analysis of harvested tissues).

Responsibilities

DUTIES & ESSENTIAL JOB FUNCTIONS

Identify the functions or tasks that employees in the job perform. The essential functions should state the purpose of the work and the results to be accomplished, rather than how the function is performed. Of the tasks listed, what percentage of time is devoted to each? The more time employees spend on a function, the more likely it is that the function is essential. Generally, include those functions that account for 10% or more of the work, i.e., key items that contribute significantly to the achievement of the job. The functions should add up to 100%.

%

of time

Essential Function (Yes/No)

Key Responsibilities

(To be completed by Supervisor)

20

Y Under supervision, perform organization and maintenance of a large, complex mouse colony (including: computerized record-keeping, breeding, monitoring, genotyping)

20

Y Under supervision, perform experimental procedures using laboratory mice: blood sampling, intravenous/intraperitoneal injection, dissection, analysis of harvested tissues

20

Y Under supervision, perform cell / Tissue culture

15

Y Under supervision, perform biochemical assays: Western blotting/ Immunoprecipitation/ Fractionation/ GAP assays

5

Y Under supervision, perform DNA and/or RNA isolation and manipulation

5

Y Under supervision, perform PCR and agarose gel electrophoresis

5

Y Under supervision, perform generation of recombinant retroviruses/lentiviruses and transduction of hematopoietic cells

5

Y Maintenance of accurate, complete and legible records

5

Y Under supervision, perform flow cytometry

100%

(To update total %, enter the amount of time in whole numbers (without the % symbol - e.g., 15, 20) then highlight the total sum (e.g., 1%) at the bottom of the column and press F9. The total sum should add up to 100%.)

Qualifications

Required Qualifications:

BA/BS in a related science and knowledge of, or experience with, the basic techniques or methods required by the position; and/or an equivalent combination of education and experience

Preferred Qualifications

Expertise in breeding and maintaining laboratory mice
Molecular biology skills including DNA/RNA extraction and PCR amplification
Tissue culture skills including passaging cell lines
Biochemistry skills including Western blotting

About UCSF

ABOUT US

The University of California, San Francisco (UCSF) is a leading university dedicated to promoting health worldwide through advanced biomedical research, graduate-level education in the life sciences and health professions, and excellence in patient care. It is the only campus in the 10-campus UC system dedicated exclusively to the health sciences. We bring together the world’s leading experts in nearly every area of health. We are home to five Nobel laureates who have advanced the understanding of cancer, neurodegenerative diseases, aging and stem cells.

Pride Values

UCSF is a diverse community made of people with many skills and talents. We seek candidates whose work experience or community service has prepared them to contribute to our commitment to professionalism, respect, integrity, diversity and excellence – also known as our PRIDE values .

In addition to our PRIDE values, UCSF is committed to equity – both in how we deliver care as well as our workforce. We are committed to building a broadly diverse community, nurturing a culture that is welcoming and supportive, and engaging diverse ideas for the provision of culturally competent education, discovery, and patient care. Additional information about UCSF is available here .

Join us to find a rewarding career contributing to improving healthcare worldwide.

Equal Employment Opportunity

The University of California is an Equal Opportunity Employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, disability, age, protected veteran status, or other protected status under state or federal law.

Salary Information

The final salary and offer components are subject to additional approvals based on UC policy.

Your placement within the salary range is dependent on a number of factors including your work experience and internal equity within this position classification at UCSF. For positions that are represented by a labor union, placement within the salary range will be guided by the rules in the collective bargaining agreement.

To learn more about the benefits of working at UCSF, including total compensation, please visit: https://ucnet.universityofcalifornia.edu/compensation-and-benefits/index.html

University of California, San Francisco

New Jobs

Staff Research Associate